Journal: PLoS ONE

Article Title: The Anti-Tumor Histone Deacetylase Inhibitor SAHA and the Natural Flavonoid Curcumin Exhibit Synergistic Neuroprotection against Amyloid-Beta Toxicity

doi: 10.1371/journal.pone.0085570

Figure Lengend Snippet: A. PC12 cells were treated with different concentrations of Aβ 25–35 (n = 5). *, p <0.05; ***, p <0.001 vs Ctrl. B. PC12 cells were treated with different concentrations of SAHA (n = 5). *, p <0.05; ***, p <0.001 vs Ctrl. C. PC12 cells were treated with different concentrations of curcumin (n = 5). *, p <0.05; ***, p <0.001 vs Ctrl. D. MTT assay was performed to detect cell viability after treating with SAHA and curcumin against Aβ 25–35 -induced cytotoxicity in PC12 cells (n = 5). ***, p <0.001 vs Ctrl; **, p <0.01 vs Aβ 25–35 .

Article Snippet: Rat pheochromocytoma PC12 cells were originally obtained from Chinapeptides Co., Ltd. PC12 neuron cells were cultured in DMEM-F12 containing 7% FBS, 1% penicillin, and 1% streptomycin at 37°C.

Techniques: MTT Assay

Journal: PLoS ONE

Article Title: The Anti-Tumor Histone Deacetylase Inhibitor SAHA and the Natural Flavonoid Curcumin Exhibit Synergistic Neuroprotection against Amyloid-Beta Toxicity

doi: 10.1371/journal.pone.0085570

Figure Lengend Snippet: A. Apoptotic cells were detected by TUNEL assay. Cells were stained with TUNEL positive nuclei (green) and nuclei of PC12 cells (blue). B. The percentage of TUNEL positive cells was determined (n = 5). ***, p <0.001 vs Ctrl; **, p <0.01 vs Aβ 25–35 . Ctrl: the control group. C. Western blot of cleaved caspase 3 (n = 3). ***, p <0.001 vs Ctrl; **, p <0.01 vs Aβ 25–35 . Ctrl: the control group; CASP3: caspase 3.

Article Snippet: Rat pheochromocytoma PC12 cells were originally obtained from Chinapeptides Co., Ltd. PC12 neuron cells were cultured in DMEM-F12 containing 7% FBS, 1% penicillin, and 1% streptomycin at 37°C.

Techniques: TUNEL Assay, Staining, Control, Western Blot

Journal: PLoS ONE

Article Title: The Anti-Tumor Histone Deacetylase Inhibitor SAHA and the Natural Flavonoid Curcumin Exhibit Synergistic Neuroprotection against Amyloid-Beta Toxicity

doi: 10.1371/journal.pone.0085570

Figure Lengend Snippet: A. Representative western blot of the p -Akt (Ser 473) protein expression with SAHA treatment (n = 3). **, p <0.01 vs Ctrl. B. Representative western blot of the p -Akt (Ser 473) protein expression with curcumin treatment (n = 3). **, p <0.01 vs Ctrl. C. Representative western blot of the p -Akt (Ser 473) protein expression with co-treatment of SAHA and curcumin (n = 3) to the PC12 cells (n = 3). *, p <0.05 vs Ctrl; **, p <0.01 vs Aβ 25–35 ; Ctrl: the control group.

Article Snippet: Rat pheochromocytoma PC12 cells were originally obtained from Chinapeptides Co., Ltd. PC12 neuron cells were cultured in DMEM-F12 containing 7% FBS, 1% penicillin, and 1% streptomycin at 37°C.

Techniques: Western Blot, Expressing, Control